Ocellated River Stingrays reproduce using an intrauterine strategy, where fully formed individuals are born. The development of the fry occurs inside the female. After they consume their yolk sacs, nutrition is provided through special filaments, which represent a primitive form of placenta. Typically, there are up to 8 fry in a single brood. River stingrays show selectivity in choosing a partner, so simply placing a male and female in the same tank is not enough for successful breeding. Natural pair formation can occur if several young fish grow together for several years. However, such a breeding strategy often exceeds the capabilities of most hobbyists. When a pair is formed, the male exhibits courtship behavior during the spawning period, pursuing the female until she is ready to mate. Then the stingrays press their ventral sides together, and the male introduces semen into the female's body using special appendages. The incubation period lasts from 9 to 12 weeks, but in aquarium conditions, this period may be shorter, which is likely due to more comfortable conditions and constant access to food.