For successful spawning of Aphyocharax nattereri, soft, slightly acidic water conditions (dGH 2–5, pH 5.5–6.0) are preferred. The fish scatter their eggs among dense aquatic plants rather haphazardly, without forming an organized clutch, so the eggs may be dispersed throughout the bottom. Despite their small size, Aphyocharax nattereri are quite prolific: one female can lay hundreds of eggs. Parental instincts are absent in this species, and the fish do not care for their offspring. Moreover, adult fish may eat their fry if given the opportunity. When planning breeding, it is recommended to move the eggs to a separate aquarium with similar water parameters. The incubation period lasts about 24 hours. In the first days of life, the fry feed on their yolk sacs, and then begin to actively search for food. Since the fry are very small, they require microscopic food, such as paramecium or special liquid/powdered fry foods.