For a group of 4–5 Otocinclus flexilis, an optimal aquarium should have a volume of 50–60 liters. Unlike other members of the genus, this species does not require an abundance of vegetation. However, the presence of tall plants, such as Vallisneria, has a beneficial effect on their keeping. Otherwise, the decor is chosen individually, based on the aquarist's preferences or the needs of other inhabitants. Long-term keeping requires maintaining clean water with suitable hydrochemical composition. It is worth noting that the wide range of permissible pH and dGH values significantly simplifies weekly aquarium maintenance, including water changes. Despite its tropical origin, this fish prefers relatively cool water, so the keeping temperature should not exceed 25°C.